1 Shri Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu, Rajasthan-333001, India.
2 Yazhini Biotech Research Laboratory, Rajapalayam-626117, Tamil Nādu, India.
3 Shri Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu, Rajasthan-333001, India.
Received on 08December 2022; revised on 19 January 2023; accepted on 21 January 2023
The three isolates were isolated from the methanolic extract of Abrus precatorius seeds by column chromatography using silica gel 60-120 mesh as the adsorbent and methanol as the mobile phase. The isolated compounds are further purified by TLC. The compounds that have the same RF value are combined. The crude extract was named CMME. The isolated compounds named CMME I, CMME II, and CMME III are first-eluted, second, eluted, and third-eluted compounds respectively. The isolated compounds were characterized by LC-MS, 1H NMR, and 13C NMR. The compound I was found to be (4,6-O-Benzylidene) methyl-alpha D-glucopyranoside, m/z282.3923, molecular formula C14H18O6. The compound II was found to be Isoflavone base + 20, O-Malonyl Hex, m/z 502.42801, molecular formula C24H22O12. The compound III was found to be 3-Carboxy-1-methyl pyridinium chloride, m/z 173.6, molecular formula C7H8ClNO2.
Column chromatography; LC-MS; 1H NMR; 13C NMR
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S Dharmaraj Santhosam, P Selvam and Abhinandan Danodia. Isolation and characterization of three isolates of Abrus precatorius seeds by LC-MS, 1HNMR, And 13CNMR. International Journal of Science and Research Archive, 2023, 08(01), 404-420. Article DOI: https://doi.org/10.30574/ijsra.2023.8.1.0056
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